LCT Kit ELISA

N° du produit ABIN2043867

Ce kit ELISA Rat LCT est un kit ELISA Colorimetric conçu pour quantifier Rat LCT.

Aperçu rapide pour LCT Kit ELISA (ABIN2043867)

Antigène: LCT (Lactase (LCT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit LCT Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.116 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Lactase (LCT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactase (LCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactase (LCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactase (LCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lactase (LCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: LCT (Lactase (LCT))