LDHD Kit ELISA

N° du produit ABIN2043869

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain LDHD.

Aperçu rapide pour LDHD Kit ELISA (ABIN2043869)

Antigène: LDHD (Lactate Dehydrogenase D (LDHD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Détail du produit LDHD Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Sensibilité: < 0.59 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Lactate Dehydrogenase D (LDHD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase D (LDHD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase D (LDHD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase D (LDHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lactate Dehydrogenase D (LDHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: LDHD (Lactate Dehydrogenase D (LDHD))

Pathways: Ribonucleoside Biosynthetic Process