LIN28A Kit ELISA
Aperçu rapide pour LIN28A Kit ELISA (ABIN2043891)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Sensibilité
- < 0.049 ng/mL
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Plaque
- Pre-coated
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Protocole
- Lin-28 Homolog A (LIN28A) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lin-28 Homolog A (LIN28A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lin-28 Homolog A (LIN28A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lin-28 Homolog A (LIN28A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lin-28 Homolog A (LIN28A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
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Autre désignation
- Lin-28 Homolog A (LIN28A)
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Pathways
- Stem Cell Maintenance
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