MAP1LC3A Kit ELISA

N° du produit ABIN2043973

Kit ELISA MAP1LC3A Humain, Colorimetric test pour la quantification de Humain MAP1LC3A.

Aperçu rapide pour MAP1LC3A Kit ELISA (ABIN2043973)

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Détail du produit MAP1LC3A Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Sensibilité: < 0.59 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Autre désignation: Microtubule Associated Protein 1 Light Chain 3 alpha (MAP1LC3a)

Pathways: Autophagy