MUC12 Kit ELISA

N° du produit ABIN2043991

Kit ELISA MUC12 Humain, Colorimetric test pour la quantification de Humain MUC12.

Aperçu rapide pour MUC12 Kit ELISA (ABIN2043991)

Antigène: MUC12 (Mucin 12, Cell Surface Associated (MUC12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit MUC12 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.132 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Mucin 12, Cell Surface Associated (MUC12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 12, Cell Surface Associated (MUC12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 12, Cell Surface Associated (MUC12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 12, Cell Surface Associated (MUC12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mucin 12, Cell Surface Associated (MUC12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: MUC12 (Mucin 12, Cell Surface Associated (MUC12))