MT-ND1 Kit ELISA

N° du produit ABIN2044037

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris MT-ND1.

Aperçu rapide pour MT-ND1 Kit ELISA (ABIN2044037)

Antigène: MT-ND1 (Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Détail du produit MT-ND1 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Sensibilité: < 0.31 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: NADH Dehydrogenase 1 (ND1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase 1 (ND1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase 1 (ND1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase 1 (ND1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of NADH Dehydrogenase 1 (ND1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: MT-ND1 (Mitochondrially Encoded NADH Dehydrogenase 1 (MT-ND1))

Autre désignation: NADH Dehydrogenase 1 (ND1)