NEFH Kit ELISA

N° du produit ABIN2044075

Kit ELISA Humain NEFH, test Colorimetric pour la quantification de Humain NEFH.

Aperçu rapide pour NEFH Kit ELISA (ABIN2044075)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62-1000 pg/mL

Application: ELISA

Détail du produit NEFH Kit ELISA

Seuil minimal de détection: 15.62 pg/mL

Sensibilité: < 5.2 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Neurofilament, Heavy Polypeptide (NEFH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Heavy Polypeptide (NEFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neurofilament, Heavy Polypeptide (NEFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))