Neutrophil Alkaline Phosphatase Kit ELISA

N° du produit ABIN2044089

Ce kit ELISA Humain Neutrophil Alkaline Phosphatase est un kit ELISA Colorimetric conçu pour quantifier Humain Neutrophil Alkaline Phosphatase.

Aperçu rapide pour Neutrophil Alkaline Phosphatase Kit ELISA (ABIN2044089)

Antigène: Neutrophil Alkaline Phosphatase (NAP)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Détail du produit Neutrophil Alkaline Phosphatase Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Sensibilité: < 0.57 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Neutrophil Alkaline Phosphatase (NAP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Alkaline Phosphatase (NAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Alkaline Phosphatase (NAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Alkaline Phosphatase (NAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neutrophil Alkaline Phosphatase (NAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Neutrophil Alkaline Phosphatase (NAP)