NKAP Kit ELISA

N° du produit ABIN2044093

Détail du produit NKAP Kit ELISA

Antigène: NKAP (NFKB Activating Protein (NKAP))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.061 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: NFKB Activating Protein (NKAP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB Activating Protein (NKAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFKB Activating Protein (NKAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB Activating Protein (NKAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of NFKB Activating Protein (NKAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: NKAP (NFKB Activating Protein (NKAP))

Abstract: NKAP Produits

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Pathways: Stem Cell Maintenance, Phosphorylation & l'infection par le SRAS-CoV-2