NCOA3 Kit ELISA

N° du produit ABIN2044127

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat NCOA3.

Aperçu rapide pour NCOA3 Kit ELISA (ABIN2044127)

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit NCOA3 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.115 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Nuclear Receptor Coactivator 3 (NCOA3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Coactivator 3 (NCOA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Receptor Coactivator 3 (NCOA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha