Osteocalcin Kit ELISA

N° du produit ABIN2044141

Kit ELISA Poulet Osteocalcin, test Colorimetric pour la quantification de Poulet Osteocalcin.

Aperçu rapide pour Osteocalcin Kit ELISA (ABIN2044141)

Antigène: Osteocalcin (BGLAP)

Reactivité: Poulet

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 pg/mL

Application: ELISA

Détail du produit Osteocalcin Kit ELISA

Seuil minimal de détection: 1.56 pg/mL

Sensibilité: < 0.67 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Osteocalcin (OC) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Osteocalcin (BGLAP)

Autre désignation: Osteocalcin (OC)