PLA2G3 Kit ELISA

N° du produit ABIN2044217

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris PLA2G3.

Aperçu rapide pour PLA2G3 Kit ELISA (ABIN2044217)

Antigène: PLA2G3 (Phospholipase A2, Group III (PLA2G3))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Détail du produit PLA2G3 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Sensibilité: < 0.27 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Phospholipase A2, Group III (PLA2G3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group III (PLA2G3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group III (PLA2G3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group III (PLA2G3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group III (PLA2G3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PLA2G3 (Phospholipase A2, Group III (PLA2G3))