WBP2NL Kit ELISA
Aperçu rapide pour WBP2NL Kit ELISA (ABIN2044253)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Sensibilité
- < 0.059 ng/mL
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Plaque
- Pre-coated
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Protocole
- Postacrosomal Sheath WW Domain Binding Protein (PAWP) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Postacrosomal Sheath WW Domain Binding Protein (PAWP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Postacrosomal Sheath WW Domain Binding Protein (PAWP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Postacrosomal Sheath WW Domain Binding Protein (PAWP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Postacrosomal Sheath WW Domain Binding Protein (PAWP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- WBP2NL (WBP2 N-terminal Like (WBP2NL))
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Autre désignation
- Postacrosomal Sheath WW Domain Binding Protein (PAWP)
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