PIP Kit ELISA
Aperçu rapide pour PIP Kit ELISA (ABIN2044283)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.62 ng/mL
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Sensibilité
- < 0.255 ng/mL
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Plaque
- Pre-coated
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Protocole
- Prolactin Induced Protein (PIP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin Induced Protein (PIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin Induced Protein (PIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin Induced Protein (PIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prolactin Induced Protein (PIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- PIP (Prolactin-Induced Protein (PIP))
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Autre désignation
- Prolactin Induced Protein (PIP)
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