PAR1 Kit ELISA

N° du produit ABIN2044311

Ce kit ELISA Rat PAR1 est un kit ELISA Colorimetric conçu pour quantifier Rat PAR1.

Aperçu rapide pour PAR1 Kit ELISA (ABIN2044311)

Antigène: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit PAR1 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.051 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Protease Activated Receptor 1 (PAR1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 1 (PAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 1 (PAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 1 (PAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease Activated Receptor 1 (PAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Autre désignation: Protease Activated Receptor 1 (PAR1)

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus