PKN1 Kit ELISA

N° du produit ABIN2044339

Détail du produit PKN1 Kit ELISA

Antigène: PKN1 (Protein Kinase N1 (PKN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Seuil minimal de détection: 0.31 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.117 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Protein Kinase N1 (PKN1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PKN1 (Protein Kinase N1 (PKN1))

Abstract: PKN1 Produits

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