PRKX Kit ELISA
Aperçu rapide pour PRKX Kit ELISA (ABIN2044345)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 1.56 ng/mL
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Marque
- BioAssay™
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Sensibilité
- < 0.60 ng/mL
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Plaque
- Pre-coated
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Protocole
- Protein Kinase, X-Linked (PRKX) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, X-Linked (PRKX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, X-Linked (PRKX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, X-Linked (PRKX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase, X-Linked (PRKX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- PRKX (Protein Kinase, X-Linked (PRKX))
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Pathways
- Thyroid Hormone Synthesis
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