PTK6 Kit ELISA
Aperçu rapide pour PTK6 Kit ELISA (ABIN2044349)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 1.56 ng/mL
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Sensibilité
- < 0.53 ng/mL
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Plaque
- Pre-coated
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Protocole
- Protein Tyrosine Kinase 6 (PTK6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Kinase 6 (PTK6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Kinase 6 (PTK6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Kinase 6 (PTK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Tyrosine Kinase 6 (PTK6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))
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Autre désignation
- Protein Tyrosine Kinase 6 (PTK6)
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