PC Kit ELISA

N° du produit ABIN2044367

Détail du produit PC Kit ELISA

Antigène: PC (Pyruvate Carboxylase (PC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.63 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Pyruvate Carboxylase (PC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Carboxylase (PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Carboxylase (PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Carboxylase (PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Carboxylase (PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: PC (Pyruvate Carboxylase (PC))

Abstract: PC Produits

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