RSPO1 Kit ELISA

N° du produit ABIN2044371

Ce kit ELISA Humain RSPO1 est un kit ELISA Colorimetric conçu pour quantifier Humain RSPO1.

Aperçu rapide pour RSPO1 Kit ELISA (ABIN2044371)

Antigène: RSPO1 (R-Spondin 1 (RSPO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62-1000 pg/mL

Application: ELISA

Détail du produit RSPO1 Kit ELISA

Seuil minimal de détection: 15.62 pg/mL

Sensibilité: < 5.8 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: R-Spondin 1 (RSPO1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 1 (RSPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 1 (RSPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 1 (RSPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of R-Spondin 1 (RSPO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: RSPO1 (R-Spondin 1 (RSPO1))