RORC Kit ELISA
Aperçu rapide pour RORC Kit ELISA (ABIN2044383)
Antigène
Voir toutes RORC Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
-
-
Seuil minimal de détection
- 0.15 ng/mL
-
Sensibilité
- < 0.054 ng/mL
-
-
-
-
Plaque
- Pre-coated
-
Protocole
- RAR Related Orphan Receptor Gamma (RORg) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAR Related Orphan Receptor Gamma (RORg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAR Related Orphan Receptor Gamma (RORg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAR Related Orphan Receptor Gamma (RORg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of RAR Related Orphan Receptor Gamma (RORg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Stock
- 4 °C/-20 °C
-
-
- RORC (RAR-Related Orphan Receptor C (RORC))
-
Autre désignation
- RAR Related Orphan Receptor gamma (RORg)
-
Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
Antigène Voir toutes RORC Kits ELISA
-