RNASE3 Kit ELISA

N° du produit ABIN2044447

Kit ELISA Humain RNASE3, test Colorimetric pour la quantification de Humain RNASE3.

Aperçu rapide pour RNASE3 Kit ELISA (ABIN2044447)

Antigène: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 39.06-2500 pg/mL

Application: ELISA

Détail du produit RNASE3 Kit ELISA

Seuil minimal de détection: 39.06 pg/mL

Sensibilité: < 17 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Ribonuclease A3 (RNASE3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A3 (RNASE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A3 (RNASE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A3 (RNASE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ribonuclease A3 (RNASE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Autre désignation: Ribonuclease A3 (RNASE3)