SPTLC2 Kit ELISA

N° du produit ABIN2044527

Détail du produit SPTLC2 Kit ELISA

Antigène: SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.055 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: SPTLC2 (serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2))

Abstract: SPTLC2 Produits

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