SIRT3 Kit ELISA

N° du produit ABIN2044563

Détail du produit SIRT3 Kit ELISA

Antigène: SIRT3 (Sirtuin 3 (SIRT3))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.055 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Sirtuin 3 (SIRT3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 3 (SIRT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sirtuin 3 (SIRT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 3 (SIRT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sirtuin 3 (SIRT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: SIRT3 (Sirtuin 3 (SIRT3))

Abstract: SIRT3 Produits

Synonymes: zgc:158774 Kit ELISA, sirtuin Kit ELISA, SIR2L3 Kit ELISA, 2310003L23Rik Kit ELISA, AI848213 Kit ELISA, Sir2l3 Kit ELISA, sirtuin 3 Kit ELISA, sirt3 Kit ELISA, SIRT3 Kit ELISA, Sirt3 Kit ELISA