SDHA Kit ELISA

N° du produit ABIN2044619

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris SDHA.

Aperçu rapide pour SDHA Kit ELISA (ABIN2044619)

Antigène: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit SDHA Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.117 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Succinate Dehydrogenase Complex Subunit A (SDHA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit A (SDHA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Succinate Dehydrogenase Complex Subunit A (SDHA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Succinate Dehydrogenase Complex Subunit A (SDHA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Succinate Dehydrogenase Complex Subunit A (SDHA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Autre désignation: Succinate Dehydrogenase Complex Subunit A (SDHA)