Superoxide Dismutases Kit ELISA

N° du produit ABIN2044629

Le kit ELISA Superoxide Dismutases (ABIN2044629) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Superoxide Dismutases.

Aperçu rapide pour Superoxide Dismutases Kit ELISA (ABIN2044629)

Antigène: Superoxide Dismutases

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.063 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Superoxide Dismutases (SOD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutases (SOD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutases (SOD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutases (SOD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Superoxide Dismutases (SOD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Superoxide Dismutases