T-Bet Kit ELISA

N° du produit ABIN2044645

Détail du produit T-Bet Kit ELISA

Antigène: T-Bet

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.052 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: T-Box Protein 21 (TBX21) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21 (TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21 (TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21 (TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of T-Box Protein 21 (TBX21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: T-Bet

Autre désignation: T-Box Protein 21 (TBX21) (T-Bet Produits)

Synonymes: T-PET Kit ELISA, T-bet Kit ELISA, TBET Kit ELISA, TBLYM Kit ELISA, TBT1 Kit ELISA, Tbet Kit ELISA, Tblym Kit ELISA, T-box 21 Kit ELISA, TBX21 Kit ELISA, Tbx21 Kit ELISA