TERT Kit ELISA

N° du produit ABIN2044655

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat TERT.

Aperçu rapide pour TERT Kit ELISA (ABIN2044655)

Antigène: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 ng/mL

Application: ELISA

Détail du produit TERT Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Sensibilité: < 1.08 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Telomerase Reverse Transcriptase (TERT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase Reverse Transcriptase (TERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomerase Reverse Transcriptase (TERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomerase Reverse Transcriptase (TERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Telomerase Reverse Transcriptase (TERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: TERT (Telomerase Reverse Transcriptase (TERT))

Pathways: Telomere Maintenance