TJP1 Kit ELISA

N° du produit ABIN2044667

Détail du produit TJP1 Kit ELISA

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.051 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Tight Junction Protein 1 (TJP1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Protein 1 (TJP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tight Junction Protein 1 (TJP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tight Junction Protein 1 (TJP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tight Junction Protein 1 (TJP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Abstract: TJP1 Produits

Synonymes: ZO-1 Kit ELISA, ZO1 Kit ELISA, zo1 Kit ELISA, tight junction protein 1 Kit ELISA, tight junction protein ZO-1 Kit ELISA, TJP1 Kit ELISA, Tjp1 Kit ELISA, LOC396567 Kit ELISA

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization