TGFB2 Kit ELISA
Aperçu rapide pour TGFB2 Kit ELISA (ABIN2044693)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 15.62 pg/mL
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Sensibilité
- < 6.5 pg/mL
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Plaque
- Pre-coated
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Protocole
- Transforming Growth Factor Beta 2 (TGFb2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 2 (TGFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 2 (TGFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 2 (TGFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transforming Growth Factor Beta 2 (TGFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
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Autre désignation
- Transforming Growth Factor beta 2 (TGFb2)
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Pathways
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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