TMEM27 Kit ELISA

N° du produit ABIN2044709

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Chien TMEM27.

Aperçu rapide pour TMEM27 Kit ELISA (ABIN2044709)

Antigène: TMEM27 (Transmembrane Protein 27 (TMEM27))

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit TMEM27 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.057 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Transmembrane Protein 27 (TMEM27) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 27 (TMEM27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 27 (TMEM27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 27 (TMEM27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Transmembrane Protein 27 (TMEM27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: TMEM27 (Transmembrane Protein 27 (TMEM27))