TNNT3A Kit ELISA
Aperçu rapide pour TNNT3A Kit ELISA (ABIN2044715)
Antigène
Tous les produits TNNT3AReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 15.62 pg/mL
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Sensibilité
- < 6.2 pg/mL
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Plaque
- Pre-coated
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Protocole
- Troponin T Type 3, Fast Skeletal (TNNT3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 3, Fast Skeletal (TNNT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin T Type 3, Fast Skeletal (TNNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 3, Fast Skeletal (TNNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Troponin T Type 3, Fast Skeletal (TNNT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- TNNT3A (Fast Skeletal Troponin T3a (TNNT3A))
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Autre désignation
- Troponin T Type 3, Fast Skeletal (TNNT3)
Antigène Tous les produits TNNT3A
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