UGT2B7 Kit ELISA
Aperçu rapide pour UGT2B7 Kit ELISA (ABIN2044749)
Antigène
Voir toutes UGT2B7 Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
-
-
Seuil minimal de détection
- 0.31 ng/mL
-
Sensibilité
- < 0.113 ng/mL
-
-
-
-
Plaque
- Pre-coated
-
Protocole
- UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Stock
- 4 °C/-20 °C
-
-
- UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7))
-
Pathways
- Steroid Hormone Biosynthesis
Antigène Voir toutes UGT2B7 Kits ELISA
-