UPRT Kit ELISA

N° du produit ABIN2044757

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain UPRT.

Aperçu rapide pour UPRT Kit ELISA (ABIN2044757)

Antigène: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit UPRT Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.051 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Uracil Phosphoribosyltransferase (UPRT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uracil Phosphoribosyltransferase (UPRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uracil Phosphoribosyltransferase (UPRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uracil Phosphoribosyltransferase (UPRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Uracil Phosphoribosyltransferase (UPRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: UPRT (Uracil phosphoribosyltransferase (FUR1) Homolog (UPRT))

Autre désignation: Uracil Phosphoribosyltransferase (UPRT)

Pathways: Ribonucleoside Biosynthetic Process