VAMP2 Kit ELISA

N° du produit ABIN2044789

Kit ELISA VAMP2 Humain, Colorimetric test pour la quantification de Humain VAMP2.

Aperçu rapide pour VAMP2 Kit ELISA (ABIN2044789)

Antigène: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit VAMP2 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.057 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Vesicle Associated Membrane Protein 2 (VAMP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicle Associated Membrane Protein 2 (VAMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vesicle Associated Membrane Protein 2 (VAMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vesicle Associated Membrane Protein 2 (VAMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vesicle Associated Membrane Protein 2 (VAMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Autre désignation: Vesicle Associated Membrane Protein 2 (VAMP2)

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport