NAMPT Kit ELISA

N° du produit ABIN2044797

Ce kit ELISA Cobaye NAMPT est un kit ELISA Colorimetric conçu pour quantifier Cobaye NAMPT.

Aperçu rapide pour NAMPT Kit ELISA (ABIN2044797)

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivité: Cobaye

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Détail du produit NAMPT Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Marque: BioAssay™

Sensibilité: < 0.52 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Visfatin (VF) BioAssay-ELISA Kit (Guinea Pig) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Autre désignation: Visfatin (VF)