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Vitamin D-Binding Protein Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat Vitamin D-Binding Protein.
N° du produit ABIN2044799

Aperçu rapide pour Vitamin D-Binding Protein Kit ELISA (ABIN2044799)

Antigène

Voir toutes Vitamin D-Binding Protein (GC) Kits ELISA
Vitamin D-Binding Protein (GC)

Reactivité

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Rat

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

12.5-800 ng/mL

Application

ELISA
  • Seuil minimal de détection

    12.5 ng/mL

    Sensibilité

    < 5.57 ng/mL
  • Plaque

    Pre-coated

    Protocole

    Vitamin D Binding Protein (DBP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Stock

    4 °C/-20 °C
  • Antigène Voir toutes Vitamin D-Binding Protein (GC) Kits ELISA

    Vitamin D-Binding Protein (GC)

    Autre désignation

    Vitamin D Binding Protein (DBP)

    Pathways

    Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process
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