Periostin Kit ELISA

N° du produit ABIN2111983

Détail du produit Periostin Kit ELISA

Antigène: Periostin (POSTN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31-2000 pg/mL

Seuil minimal de détection: 31 pg/mL

Application: ELISA

Marque: BioAssay™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma

Analytical Method: Quantitative

Sensibilité: 10 pg/mL

Ingrédients: Mouse periostin Standard, lyophilized, 1x5ug
Detection Antibody, 1x20 µL
HRP Labeled Streptavidin, lyophilized, 1x2ug
Wash Buffer 10X, 2x30 mL
ELISA Buffer 10X, 2x30 mL
TMB Substrate Solution, 1x10 mL
Stop Solution, 1x10 mL
Plate coated with periostin antibody, 6x16 well strips
Plate covers, 2x plastic film
Silica gel, 2x minibags

Information d'application

Plaque: Pre-coated

Protocole: This BioAssay-is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3-,5,5-- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of periostin in the samples.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Periostin (POSTN)

Autre désignation: Periostin (POSTN Produits)

Synonymes: fa99h07 Kit ELISA, pn Kit ELISA, postn Kit ELISA, wu:fa99h07 Kit ELISA, wu:fc70f09 Kit ELISA, zgc:153873 Kit ELISA, LOC100008945 Kit ELISA, osf-2 Kit ELISA, pdlpostn Kit ELISA, periostin Kit ELISA, OSF-2 Kit ELISA, OSF2 Kit ELISA, PDLPOSTN Kit ELISA, PN Kit ELISA, RP11-412K4.1 Kit ELISA, A630052E07Rik Kit ELISA, AI747096 Kit ELISA, Osf2 Kit ELISA, PLF Kit ELISA, peri Kit ELISA, Plf Kit ELISA, periostin Kit ELISA, periostin, osteoblast specific factor b Kit ELISA, periostin, osteoblast specific factor Kit ELISA, POSTN Kit ELISA, postnb Kit ELISA, postn Kit ELISA, Postn Kit ELISA

Sujet: Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein 2 (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.