Hepcidin Kit ELISA

N° du produit ABIN2111986

Kit ELISA Hepcidin Porc, Colorimetric test pour la quantification de Porc Hepcidin.

Aperçu rapide pour Hepcidin Kit ELISA (ABIN2111986)

Antigène: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 3.7-300 ng/mL

Application: ELISA

Détail du produit Hepcidin Kit ELISA

Seuil minimal de détection: 3.7 ng/mL

Analytical Method: Quantitative

Sensibilité: < 1.48 ng/mL

Ingrédients: A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30X, 1x20 mL

Information d'application

Plaque: Pre-coated

Protocole: The Porcine Hepcidin (Hepc) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of Hepc in porcine serum, plasma, tissue homogenates, cell lysates, culture supernatants and other biological fluids.This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for Hepc has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled Hepc and unlabeled Hepc (standards or samples) for limited binding sites on the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of Hepc in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Hepc in the sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Autre désignation: Hepcidin (Hepc)

Pathways: Hormone Activity, Transition Metal Ion Homeostasis