Thrombomodulin Kit ELISA

N° du produit ABIN2648546

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Thrombomodulin.

Aperçu rapide pour Thrombomodulin Kit ELISA (ABIN2648546)

Antigène: Thrombomodulin (THBD)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit Thrombomodulin Kit ELISA

Fonction: sCD141 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD141 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD141.

Analytical Method: Qualitative and Quantitative

Sensibilité: 0.31 ng/ml

Attributs du produit: Human sCD141 ELISA Assay kit is for Research Use Only.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 1 - 2 h

Plaque: Pre-coated

Protocole: A capture Antibody highly specific for CD141 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of CD141 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-CD141 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of CD141 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of CD141 in any sample tested.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Thrombomodulin (THBD)

Autre désignation: CD141 / THROMBOMODULIN