Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA) Kit ELISA

N° du produit ABIN2648603

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody.

Aperçu rapide pour Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA) Kit ELISA (ABIN2648603)

Antigène: Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA)

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma

Détail du produit

Sensibilité: 0.13 AU/mL

Attributs du produit: Anti-neutrophilic-cytoplasm antibodies (ANCA) represents a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods. With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 10 μL

Durée du test: 1.5 h

Protocole: Anti PR3 (c-ANCA) ELISA Assay Kit is a test is based on the binding of the antibodies in the sample with human neutrophil proteinase 3 coated into the microplates. In the first step the antibodies in calibrators, controls or prediluted patient samples bind to the inner surface of the wells. After a 60 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. In the second step an anti-human-IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized antigens. After a 30 minutes incubation any excess enzyme conjugate which is not specifically bound is washed away with the wash buffer.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Proteinase 3 Anti-Neutrophil Cytoplasmic Antibody (PR3 cANCA)

Autre désignation: ANTI PR-3 (C-ANCA)

Classe de substances: Antibody