Dopamine Kit ELISA
Aperçu rapide pour Dopamine Kit ELISA (ABIN2648624)
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Méthode de détection
Application
Type d'échantillon
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Sensibilité
- Urine: 0.46 ng/ml, Plasma: 23 pg/ml
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Attributs du produit
- Catecholamine is the name of a group of aromatic amines (noradrenaline, adrenaline, dopamine, and their derivatives) which act as hormones and neurotransmitter, respectively. Adrenaline and noradrenaline are formed from dopamine. They act on the cardiac musculature and the metabolism (adrenaline) as well as on the peripheral circulation (noradrenaline) and help the body to cope with acute and chronic stress.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 15 μL
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Plaque
- Pre-coated
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Protocole
- The competitive Dopamine ELISA kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated dopamine from the sample and solid phase bound dopamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase dopamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase dopamine is inversely proportional to the dopamine concentration of the sample.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- Dopamine (DA)
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Autre désignation
- Dopamine
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Classe de substances
- Chemical
Antigène Voir toutes Dopamine (DA) Kits ELISA
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