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Histamine Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Histamine.
N° du produit ABIN2648672

Aperçu rapide pour Histamine Kit ELISA (ABIN2648672)

Antigène

Voir toutes Histamine (HIS) Kits ELISA
Histamine (HIS)

Reactivité

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Humain

Méthode de détection

Colorimetric

Application

ELISA

Type d'échantillon

Plasma, Urine, Cell Culture Cells
  • Sensibilité

    0.02 ng/mL

    Attributs du produit

    Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Volume d'échantillon

    50 μL

    Durée du test

    2.5 h

    Plaque

    Pre-coated

    Protocole

    The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

    Restrictions

    For Research Use only
  • Stock

    4 °C
  • Antigène Voir toutes Histamine (HIS) Kits ELISA

    Histamine (HIS)

    Autre désignation

    Histamine

    Classe de substances

    Chemical
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