Histamine Kit ELISA

N° du produit ABIN2648672

Détail du produit Histamine Kit ELISA

Antigène: Histamine (HIS)

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Plasma, Urine, Cell Culture Cells

Sensibilité: 0.02 ng/mL

Attributs du produit: Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 50 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Histamine (HIS)

Autre désignation: Histamine (HIS Produits)

Classe de substances: Chemical