Insulin Autoantibody Kit ELISA

N° du produit ABIN2648679

Le kit ELISA Insulin Autoantibody (ABIN2648679) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Insulin Autoantibody.

Aperçu rapide pour Insulin Autoantibody Kit ELISA (ABIN2648679)

Antigène: Insulin Autoantibody

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum

Détail du produit

Sensibilité: 0.08 U/mL

Attributs du produit: Type 1 diabetes, also known as insulin-dependent diabetes mellitus (IDDM), results from a chronic autoimmune destruction of the insulin-secreting pancreatic beta cells, probably initiated by exposure of genetically susceptible host to an environmental agent. Autoimmune destruction of beta cells is thought to be completely asymptomatic until 80-90 % of the cells are lost. This process may take years to complete and may occur at any time in all ages.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 5 μL

Durée du test: 1.5 h

Plaque: Pre-coated

Protocole: In the first step Insulin AAb from the diluted sample bind to human recombinant insulin coated on the microtiter plate. After an incubation of 60 minutes at 37 °C unbound components are removed by washing. In a next step bound antibodies reacts with the added anti-human-IgG horseradish peroxidase (HRP) complex. Excessive conjugate is removed after 15 minutes at 37 °C by another washing step. HRP converts the colorless substrate TMB added into a blue product. The enzyme reaction is stopped by adding an acid solution after 15 minutes at 37 °C. The absorbance of the resulting yellow product is measured at 450 / 620 nm within 30 minutes. The obtained OD is direct proportional to the amount of bound antibodies.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Insulin Autoantibody

Autre désignation: IAA (Insulin Autoantibodies)

Classe de substances: Antibody