IL-10 Kit ELISA
Aperçu rapide pour IL-10 Kit ELISA (ABIN2648750)
Antigène
Voir toutes IL-10 (IL10) Kits ELISAReactivité
Méthode de détection
Application
Type d'échantillon
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Fonction
- Mouse Interleukin 10 (IL-10) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interleukin 10 (IL-10) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensibilité
- 7 pg/ml
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Attributs du produit
- Mouse Interleukin 10 (IL-10) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 10 (IL10) ELISA Kit
IL10 Reactivité: Rat Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Humain Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Souris AA 19-178 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Humain AA 19-178 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Rat AA 19-178 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Souris Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Humain Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Ascitic Fluid, Cell Culture Supernatant, Cerebrospinal Fluid, Saliva, Serum, Urine
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Humain Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Porc Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 Reactivité: Lapin Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Durée du test
- 3.5 h
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Plaque
- Pre-coated
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Protocole
- Mouse Interleukin 10 (IL-10) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-10 is added to the wells and binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IL-10 (IL10) (Interleukin 10 (IL10))
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Autre désignation
- IL-10
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
Antigène Voir toutes IL-10 (IL10) Kits ELISA
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