IL-10 Kit ELISA

N° du produit ABIN2648753

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Porc IL-10.

Aperçu rapide pour IL-10 Kit ELISA (ABIN2648753)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité: Porc

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit IL-10 Kit ELISA

Sensibilité: 7 pg/ml

Attributs du produit: Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-4). All IL-10 family members are secreted α-helical proteins. Porcine IL-10 is a secreted, possibly glycosylated, polypeptide with an 18 kDa molecular weight (5). Based on human studies, porcine IL-10 is likely to circulate as a nondisulfide-linked homodimer (3). Porcine IL-10 is synthesized as a 175 amino acid (aa) precursor with an 18 aa signal sequence and a 157 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which form two intrachain disulfide bridges (6). Mature porcine IL-10 shows 71 % , 70 %, 76 %, 75 %, 77 %, and 71 % aa sequence identity to rat (7), mouse (8), human (9), guinea pig (10), canine (11), and cotton rat (12) IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells (13), cytotoxic CD8+ T cells secreting Th2-like cytokines (14), CD4+CD45RA- (memory) Th1 and Th2 cells (15), macrophages (16), monocytes (17), CD5+ and CD5- B cells (18, 19), dendritic cells (20, 21), hepatic stellate (Ito cells) (22), keratinocytes (23), melanoma cells (24), mast cells (25), placental cytotrophoblasts (26), and fetal erythroblasts (27).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: Swine Interleukin 10 (IL-10) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any IL-10 present is bound by the immobilized antibody and the detection antibody specific for IL-10 is binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL-10

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints