Myeloperoxidase Kit ELISA

N° du produit ABIN2648790

Ce kit ELISA Humain Myeloperoxidase est un kit ELISA Colorimetric conçu pour quantifier Humain Myeloperoxidase.

Aperçu rapide pour Myeloperoxidase Kit ELISA (ABIN2648790)

Antigène: Myeloperoxidase (MPO)

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma

Détail du produit Myeloperoxidase Kit ELISA

Fonction: Human Myeloperoxidase (MPO) Serum/Plasma ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human myeloperoxidase (MPO) levels in serum and EDTA-plasma samples. The test is useful for detecting elevated levels of myeloperoxidase in serum and plasma samples.

Analytical Method: Quantitative

Sensibilité: .65 ng/ml

Attributs du produit: Human Myeloperoxidase (MPO) Serum/Plasma ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: Human Myeloperoxidase (MPO) Serum/Plasma ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human myeloperoxidase in serum and plasma samples. The assay utilizes the two-site "sandwich" technique with selected antibodies that bind to different epitopes of myeloperoxidase Assay standards, controls and diluted patient samples are added directly to wells of a microtiter plate that is coated with antibody to myeloperoxidase. After an incubation period, the plate is washed and horseradish peroxidase (HRP) conjugated human myeloperoxidase antibody is added to each well. After the second incubation period, a "sandwich" of solid-phase monoclonal antibody - human myeloperoxidase - HRP conjugated antibody" is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human myeloperoxidase in the test sample. A standard curve is generated by plotting the absorbance versus the respective human myeloperoxidase concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human myeloperoxidase in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Myeloperoxidase (MPO)

Pathways: Chromatin Binding