Normetanephrine & Metanephrine Kit ELISA

N° du produit ABIN2648796

Le kit ELISA Normetanephrine & Metanephrine (ABIN2648796) Chemical est un kit ELISA Colorimetric conçu pour quantifier Chemical Normetanephrine & Metanephrine.

Aperçu rapide pour Normetanephrine & Metanephrine Kit ELISA (ABIN2648796)

Antigène: Normetanephrine & Metanephrine

Reactivité: Chemical

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Plasma (EDTA)

Détail du produit

Fonction: The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma.

Analytical Method: Quantitative

Sensibilité: Metanephrine: 10 pg/ml, Normetanephrine: 15 pg/ml

Attributs du produit: Normetanephrine and metanephrine are physiologically formed from the catechol-amines noradrenaline and adrenaline by the enzyme catechol-O-methyltransferase (COMT). Increased levels of normetanephrine and metanephrine can be found in pheochromocytoma, ganglioneuroma and other neurogenic tumors. The Normetanephrine & Metanephrine (Plasma) ELISA assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in EDTA plasma. Normetanephrine and metanephrine are quantitatively acylated to their N-acyl-derivates.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 400 μL

Protocole: The competitive Normetanephrine & Metanephrine (Plasma) ELISA Kit uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. Acylated nor-/metanephrines from the sample and solid phase bound nor-/metanephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase nor-/metanephrine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Normetanephrine & Metanephrine