MMP7 Kit ELISA

N° du produit ABIN2681244

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain MMP7.

Aperçu rapide pour MMP7 Kit ELISA (ABIN2681244)

Antigène: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.9 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Supernatant

Détail du produit MMP7 Kit ELISA

Seuil minimal de détection: 4.9 pg/mL

Fonction: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytical Method: Quantitative

Specificité: Human MMP-7 ELISA Kit

Ingrédients:

• Plate
• Standard
• Diluent

Matériel non inclus: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: protect your eyes

Stock: 4 °C

Stockage commentaire: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Détail du antigène

Antigène: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Autre désignation: MMP7

Sujet: Matrix metalloproteinase-7 (MMP-7) also known as matrilysin, pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme that in humans is encoded by the MMP7gene. MMP-7 are commonly expressed in epithelial cells including ductal epithelium of exocrine glands in skin, salivary glands, pancreas, glandular epithelium of intestine and reproductive organ, liver, and breast. In addition, MMP7 is highly expressed in the luminal surface of dysplastic glands in human colorectal cancers. The expression of MMP7 is regulated by the Wnt/β catenin signaling pathway, and mediated by transformation growth factor β (TGF-β). The primary role of cleaved/activated MMP7 is to break down extracellular matrix by degrading macromolecules including casein, type I, II, IV, and V gelatins, fibronectin, and proteoglycan. The activated MMP-7 can cleave the propeptides of proMMP-2 and proMMP-9 to facilitate tumor invasion.

NCBI Accession: NM_002423

UniProt: P09237

Pathways: Production of Molecular Mediator of Immune Response